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为探明东方百合茎腐病的发病机制及抗病机理,以湖南株洲地区的东方百合为材料,分别采用升汞消毒、刺伤浸苗接种、超声波甲醇浸提、混液平板等方法对其病原菌进行了分离、抗病性鉴定、皂苷含量及活性测定。结果显示:尖孢镰刀菌Fusarium oxysporum为湖南株洲地区东方百合茎腐病病原菌;30份自主培育的东方百合材料通过聚类分析可划分为3类,包含抗病材料3份、中抗材料16份、感病材料11份,其中y、ZN-12和ZN-27的抗病指数都在65.00以上;抗病材料的总皂苷含量明显高于感病材料,且皂苷含量与抗性水平之间极显著相关;总皂苷含量为1.194mg/mL的提取液对茎腐病病原菌菌丝生长的抑制率为29.39%。表明y、ZN-12和ZN-27具有较好的开发潜力;总皂苷含量与东方百合抗茎腐病水平密切相关,可作为大规模筛选抗病材料的一个重要指标。
In order to find out the pathogenesis and disease resistance mechanism of oriental lily stalk rot, the oriental lily of Zhuzhou area in Hunan Province was used as material to detect the pathogen of the lily stalk disease by using mercuric chloride disinfection, stabbing seedling inoculation, ultrasonic extraction of methanol, Isolation, disease resistance identification, saponin content and activity determination. The results showed that Fusarium oxysporum was the pathogen of stalk rot of Oriental lily in Zhuzhou, Hunan. Thirty species of independently cultivated oriental lily were divided into three groups according to cluster analysis, including 3 disease-resistant materials, 16 medium- , And 11 susceptible materials, of which the resistance index of y, ZN-12 and ZN-27 were over 65.00. The total saponin content of disease-resistant materials was significantly higher than that of susceptible materials, and the relationship between the content of saponin and the resistance level Significantly related to the total saponin content of 1.194mg / mL of the extract on the stalk pathogen mycelial growth inhibition rate of 29.39%. The results showed that y, ZN-12 and ZN-27 had good development potential. The content of total saponins was closely related to the resistance to stem rot of Oriental lily, which could be used as an important index for large-scale screening of disease-resistant materials.