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在三硝基甲苯(TNT)最终浓度为 0.04、0.2与 1.0 mM的大鼠肝线粒体、微粒体测试系统中,肝微粒体超氧阴离子(O_2~+)的产生显著增加;肝线粒体O_2~+的含量除0.04mMTNT 浓度组外,其它各组的含量显著高于对照组。在TNT 浓度为2、100与200mM 时过氧化氢(H_2O_2)含量也显著增加,并以线粒体的H_2O_2增加更为明显。TNT 诱发大鼠肝线粒体、微粒体的 O_2~+和H_2O_2 产生皆有明显的时相过程。与阳性对照组Benzyl Viologen 相比,二者诱发O_2~+生成的作用在微粒体无显著差异;但在线粒体中,TNT 的诱发作用远大于Benzyl Viologen,提示TNT经还原活化使机体处于较高的氧化性应激状态。
The production of superoxide anion (O 2 +) in liver microsomes was significantly increased in the rat liver mitochondria and microsome test system with the concentrations of trinitrotoluene (TNT) of 0.04, 0.2 and 1.0 mM. The mitochondrial O 2 + In addition to 0.04mMTNT concentration group, the other groups were significantly higher than the control group. H 2 O 2 content increased significantly at 2,100 and 200 mM concentrations of TNT, with the increase of mitochondrial H 2 O 2 being more obvious. TNT induced rat liver mitochondria, microsomal O 2 ~ + and H 2 O 2 production are obvious phase process. Compared with the positive control group, Benzyl Viologen, the induction of O 2 + production in the two groups showed no significant difference in microsomes. However, in mitochondria, the induction of TNT was greater than that of Benzyl Viologen, suggesting that the reduction of TNT leads to a higher body Oxidative stress.