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目的探讨再生障碍性贫血(AA)患者脐血输注治疗前后细胞因子水平的变化。方法用ELISA法检测脐血输注前后20例AA患者骨髓及外周血中白细胞介素(IL)2、IL-3I、L-11、促血小板生成素(TPO)、干扰素γ(IFN-γ)水平变化。结果与输注前比较,脐血输注后AA患者外周血中IL-2、IFN-γ水平降低[(127.40±79.83)pg/ml vs.(90.77±4.86)pg/ml、(9.86±5.35)pg/ml vs.(4.48±3.35)pg/ml](P<0.05),IL-3水平升高[(99.13±55.31)pg/ml vs.(141.22±33.13)pg/ml)](P<0.05);脐血输注后,AA患者骨髓IL-2水平明显降低[(40.20±17.40)pg/ml vs.(5.87±0.96)pg/ml](P<0.05)。结论脐血输注可能通过改变造血环境中细胞因子的浓度或比例改善AA患者的造血功能。
Objective To investigate the changes of cytokines levels before and after cord blood transfusion in patients with aplastic anemia (AA). Methods The levels of interleukin (IL) 2, IL-3I, L-11, TPO and IFN-γ in bone marrow and peripheral blood of 20 AA patients before and after umbilical cord blood transfusion were detected by ELISA. Change in level. Results Compared with those before transfusion, the levels of IL-2 and IFN-γ in the peripheral blood of AA patients after cord blood transfusion were decreased [(127.40 ± 79.83) pg / ml vs. (90.77 ± 4.86) pg / ml, (P <0.05), IL-3 level increased (99.13 ± 55.31) pg / ml vs. (141.22 ± 33.13) pg / ml) <0.05). After cord blood transfusion, the level of IL-2 in bone marrow of AA patients was significantly lower than that of AA patients [(40.20 ± 17.40) pg / ml vs. (5.87 ± 0.96) pg / ml] Conclusion Umbilical cord blood transfusion may improve hematopoietic function in patients with AA by changing the concentration or proportion of cytokines in the hematopoietic environment.