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目的:探讨雷帕霉素(rapamycin,RAPA)在体外对胰腺癌细胞株SW1990生长和凋亡的影响及其可能的机制。方法:用5、10、20、30、40和50nmol/L RAPA作用SW1990细胞24、48和72h,MTT法检测细胞的生长抑制率,FCM法检测细胞凋亡和细胞周期,Western印迹法检测SW1990细胞中bcl-2、bcl-xL、bax和survivin蛋白的表达。结果:MTT和FCM检测结果显示,随着RAPA浓度的增加和作用时间的延长,SW1990细胞的生长抑制率和细胞凋亡率逐渐增加,与对照组比较,差异有统计学意义(P<0.05);各浓度RAPA作用细胞24h后,随着浓度的增加,G0/G1期细胞逐渐增多,S期和G2/M期细胞逐渐减少,细胞阻滞于G1期,与对照组比较,差异有统计学意义(P<0.05);Western印迹法检测结果显示,50nmol/L RAPA作用SW1990细胞后,bax表达明显增加,bcl-2、bcl-xL、survivin、bcl-xL/bax和bcl-2/bax表达明显降低,差异有统计学意义(P<0.05)。结论:RAPA可显著抑制SW1990细胞增殖和诱导细胞凋亡,其机制可能与凋亡调控基因bcl-2、bcl-xL、bax和survivin表达水平变化有关。
Objective: To investigate the effect of rapamycin (RAPA) on the growth and apoptosis of pancreatic cancer cell line SW1990 in vitro and its possible mechanism. Methods: The SW1990 cells were treated with 5, 10, 20, 30, 40 and 50 nmol / L RAPA for 24,48 and 72 h respectively. The growth inhibition rates of SW1990 cells were detected by MTT assay. Cell apoptosis and cell cycle were detected by FCM. The expression of bcl-2, bcl-xL, bax and survivin in cells. Results: The results of MTT and FCM showed that the growth inhibition rate and apoptosis rate of SW1990 cells increased gradually with the increase of RAPA concentration and the extension of time. Compared with the control group, the difference was statistically significant (P <0.05) ; After treated with various concentrations of RAPA for 24 h, the cells in G0 / G1 phase increased gradually, the cells in S phase and G2 / M phase decreased gradually, and the cell arrested in G1 phase with the increase of concentration, the difference was statistically (P <0.05). Western blotting showed that the expression of bax, bcl-2, bcl-xL, survivin, bcl-xL / bax and bcl-2 / bax in SW1990 cells treated with 50nmol / L RAPA were significantly increased Significantly lower, the difference was statistically significant (P <0.05). CONCLUSION: RAPA can significantly inhibit the proliferation and induce the apoptosis of SW1990 cells. The mechanism may be related to the changes of the expression of bcl-2, bcl-xL, bax and survivin.