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[目的]探讨腹腔注射四氯化碳(CCl4)辅以饮食调控制备猪肝硬化模型的可行性。[方法]以普通猪作为实验对象,腹腔内注射40%CCl4脂肪乳溶液0.25 ml/kg,1周2次,以玉米面为唯一饲料,5%乙醇作饮料。观察一般情况,监测门静脉压,间断采血检测肝功能、清蛋白(Alb)、总胆红素(Tbil)等指标;定期切取小块肝组织作苏木精-伊红染色和VG染色。[结果]9~10周后,肝硬化形成率83.3%,死亡率33.3%;丙氨酸氨基转移酶(ALT)、门冬氨酸转氨酶(AST)早期呈逐渐上升趋势,后期ALT、AST趋于平稳并有下降的趋势;Tbil持续上升;Alb持续下降;门静脉压早期呈下降趋势,后期迅速上升。[结论]肝硬化模型成功率较高,造模时间短,死亡率较低;病理活检仍是目前确诊肝硬化的金标准;Tbil、Alb水平在肝硬化形成不同阶段差异具有统计学意义。
[Objective] To investigate the feasibility of intraperitoneal injection of carbon tetrachloride (CCl4) supplemented with dietary regulation to prepare model of liver cirrhosis. [Method] With ordinary pig as experiment object, intraperitoneal injection of 40% CCl4 fat emulsion 0.25 ml / kg, twice a week, using cornmeal as sole feed and 5% ethanol as beverage. The general conditions were observed. The portal venous pressure and intermittent blood samples were collected to detect the liver function, albumin (Alb), total bilirubin (Tbil) and other indicators. Small pieces of liver tissue were collected for hematoxylin-eosin staining and VG staining. [Results] The rate of formation of liver cirrhosis was 83.3% and the mortality rate was 33.3% after 9 to 10 weeks. The levels of ALT and AST were gradually increased in the early stage, In a steady and declining trend; Tbil continued to rise; Alb continued to decline; portal pressure decreased early trend, the latter rose rapidly. [Conclusion] The success rate of liver cirrhosis model is high, the modeling time is short and the mortality rate is low. The pathological biopsy is still the gold standard for the diagnosis of cirrhosis. The difference of Tbil and Alb levels in different stages of cirrhosis is statistically significant.