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本文研究了Se、VA和VE及其联合作用对人类乳腺癌细胞BCaP-37增殖的影响,结果提示Se对该细胞的增殖有双向作用,浓度高于5μM抑制细胞生长,低于该浓度则刺激细胞生长。醋酸维生素A酯浓度在1.0—10.0mg/L范围内对该细胞生长有明显的抑制。维生素A酸对该细胞的生长仅有很弱的抑制作用。VE对细胞也有弱抑制生长的作用。在稍高于生理浓度的水平下,Se(5μm)与VA(2mg/L)和VE(20mg/L)三者联合处理细胞,细胞核酸含量明显降低,提示Se、VA和VE能协同抑制BCaP-37细胞的增殖,而且这种抑制有可逆的趋势。联合使用Se和VA或VE未观察到二者间对细胞生长有协同抑制。上述结果表明联合使用Se、VA和VE对乳腺癌的防治可能是有益的。
This study investigated the effects of Se, VA, and VE and their combined effects on the proliferation of human breast cancer cells, BCaP-37, suggesting that Se has a two-way effect on the proliferation of these cells. Concentration higher than 5 μM inhibits cell growth, below which stimulation stimulates Cell growth. The concentration of vitamin A acetate in the range of 1.0-10.0 mg/L significantly inhibited the growth of the cells. Vitamin A acid has only a very weak inhibitory effect on the growth of this cell. VE also weakly inhibits the growth of cells. At a slightly higher physiological level, the combination of Se (5 μm), VA (2 mg/L), and VE (20 mg/L) significantly reduced the cellular nucleic acid content, suggesting that Se, VA, and VE could synergistically inhibit BCaP. -37 cell proliferation, and this inhibition has a reversible trend. No synergistic inhibition of cell growth was observed between Se and VA or VE. The above results suggest that the combined use of Se, VA, and VE may be beneficial for the prevention and treatment of breast cancer.