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经3年研究建立了一套用离体稻叶检测稻种带细条病菌的方法.应用该方法成功地将44份自然发病的细条病病种接种到离体稻叶上并产生菌脓.用1%水琼脂加90 ppm元毒苯?咪唑,在30℃下可使水稻幼苗的离体叶保持15～20 d,可满足细条病接种发病的时间需要.应用纯菌测定该方法的灵敏度,最低可检测浓度为5×10~3Cells/ml.由于选择了最佳的温度、湿度、光照、苗龄及其它有关环境条件,使1.2～4.0g的病种经研碎浸泡后浓缩针刺接种于离体稻叶上在3 d后出现病斑,5.5～8.5 d后产生菌脓.与其它血清学检测方法相比,离体检测法具有直观、可靠、简便、稳定等优点,特别可避免由于种子携带丧失活性的细条病菌或其它病菌而引起的误诊.
After 3 years of research, a set of in vitro rice leaf test was used to detect the pathogenic bacteria in the rice seedbed.Using this method, 44 naturally occurring filamentous seedlings were inoculated on the in vitro rice leaves and pus were produced.Using 1 % Water agar with 90 ppm of TDB can keep the isolated leaves of rice seedlings for 15-20 d at 30 ℃, which can meet the need of the time of vaccination of the stripe virus. The sensitivity of this method is the lowest Detectable concentration of 5 × 10 ~ 3Cells / ml.Because of the best choice of temperature, humidity, light, age and other environmental conditions, 1.2 ~ 4.0g of the disease after grinding the seed soaking concentrated Compared with other serological detection methods, the in vitro detection method has the advantages of being intuitive, reliable, simple and stable, etc. In particular, it is possible to avoid Misdiagnosis of seeds caused by inactivation of Bacillus spp. Or other germs.