论文部分内容阅读
目的检测苯中毒信号转导相关基因的表达,探讨苯引起的血液系统损害的发病机理。方法应用含4265条信号转导基因的cDNA芯片检测7例苯中毒患者和7例无苯接触的正常人的外周血白细胞基因表达谱,观察其在基因表达谱上的差异。进行了基因表达谱聚类分析。结果在4265条基因中,存在明显差异表达的基因176条。至少在六张芯片结果中有显著性表达上调的基因35个,主要包括有PTPRC、STAT4、IFITM1等。至少在5张芯片结果中有显著性表达下调的基因45个,主要包括有ARHB、PPP3CB、CDC37等。结论微矩阵基因芯片在检测苯中毒信号转导相关基因的改变时,具有快速、高通量、高灵敏度等特点,细胞信号转导的障碍在苯中毒发病中起一定的作用。
Objective To detect the expression of benzene poisoning signal transduction related genes and to explore the pathogenesis of benzene-induced hematological damage. Methods The cDNA microarray containing 4265 signal transduction genes was used to detect the gene expression profiles of peripheral leukocytes in seven benzene poisoning patients and seven normal controls without benzene exposure. The differences in gene expression profile were observed. Gene cluster analysis was carried out. Results Among the 4265 genes, 176 genes were significantly differentially expressed. There were 35 genes with significant upregulation in at least six chip results, including PTPRC, STAT4, IFITM1 and so on. There are 45 genes with significant expression down-regulation in at least 5 chip results, including ARHB, PPP3CB and CDC37. Conclusion The microarray microarray has the characteristics of rapid, high-throughput and high sensitivity in the detection of the gene related to signal transduction of benzene poisoning. The obstacle of cell signal transduction plays a certain role in the pathogenesis of benzene poisoning.