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以冬性四倍体硬粒小麦(Triticum durum,2n=28,AABB)为母本与粗山羊草(Aegilops tauschii,2n=14,DD)杂交,得到的单倍体幼胚(n=21,ABD)经组织培养拯救,获得的幼苗经染色体加倍而成为合成小麦(AABBDD)。从中鉴定、筛选出冬性的合成小麦。幼胚仅在1/2 MS培养基上培养,成苗率为75.81%;根据幼胚的发育状态,将发育较完善的幼胚直接接种在1/2 MS培养基上,将发育不良的幼胚先接种于1/2 MS+2 mg/L 2,4-D培养基上进一步养育幼胚,之后视幼胚发育状况再将其转入1/2 MS培养基中培养成苗,此方法的成苗率为92.44%,较前者的成苗率提高了16.63%。染色体加倍在冬季塑膜拱棚内用0.05%秋水仙素进行半根法处理,较容易获得健壮苗,并且分蘖多。
The haploid immature embryos (n = 21, 20, 20, 20, 20, 20) were obtained by crossing Triticum durum (2n = 28, AABB) with Aegilops tauschii ABD) was rescued by tissue culture and the seedlings obtained were doubled to become synthetic wheat (AABBDD) by chromosomes. From identification, screening of winter synthetic wheat. The immature embryos were only cultured on ½ MS medium and the rate of seedling was 75.81%. According to the developmental state of immature embryos, immature embryos with better development were directly inoculated on 1/2 MS medium, Embryos were first inoculated on 1/2 MS + 2 mg / L 2,4-D medium to further immature embryos, and then transferred into 1/2 MS medium according to the development of the immature embryo culture seedlings, this method Of the seedling rate was 92.44%, compared with the former seedling rate increased by 16.63%. Chromosome doubling in winter plastic film shed with 0.05% colchicine half root treatment, easier to get healthy seedlings, and more tillers.