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4-香豆酸辅酶A连接酶(4CL)是位于苯丙烷类共同代谢途径中类黄酮、木质素以及肉桂酸酯等分支代谢途径交叉点上的关键酶,对整个代谢过程起着重要的调控作用。4CL基因的表达可被真菌侵染,紫外辐射,机械损伤等外界刺激所诱导。笔者将水稻R4CL-1基因启动子与GUS基因融合导入烟草基因组中,并在紫外诱导下对转基因植株进行了β-Glucuronidase活性的组织化学染色分析。在转基因烟草植株幼叶的叶脉及其周围细胞、幼根的根尖细胞及维管组织、成熟花的花瓣及柱头、未成熟种子的表皮细胞和成熟种子的胚中都检测到了GUS活性,而在完全伸展的老叶、茎(幼茎和成熟茎)、老根和花的其它组织中均没有观察到GUS酶活性,从而证明了R4CL-1启动子能在植物发育过程中指导GUS报告基因的组织特异性表达。
4-coumarate Coenzyme A ligase (4CL) is a key enzyme located at the intersection of metabolic pathways such as flavonoids, lignin, and cinnamate in the common metabolic pathway of phenylpropanoids, and plays an important regulatory role in the entire metabolic process effect. 4CL gene expression can be fungal infection, ultraviolet radiation, mechanical damage induced by external stimuli. The promoter of rice R4CL-1 gene was fused with GUS gene into tobacco genome, and the transgenic plants were analyzed by histochemical staining for β-Glucuronidase activity under ultraviolet light. GUS activity was detected in embryos and surrounding cells of young leaves of transgenic tobacco plants, root tips and vascular tissues of young roots, petal and stigma of mature flowers, epidermal cells of immature seeds and mature embryos No GUS enzyme activity was observed in fully expanded old leaves, stems (young and mature stems), root and other tissues of flowers, demonstrating that the R4CL-1 promoter can direct the GUS reporter gene during plant development Tissue-specific expression.