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钙在心肌细胞的各种生理生化反应和疾病发生、发展中起重要作用,心肌细胞钙代谢的研究是目前医学研究中的重点之一。用荧光法恻定活细胞胞浆内游离钙([Ca~(2+)]i)的变化是近年来兴起的一门新技术。尚未见国内用上述方法测定分离的成年大鼠心肌细胞胞浆内[Ca~(2+)]i均报道。本试验目的在于建立该方法(Fura-2为
Calcium in cardiomyocytes in a variety of physiological and biochemical reactions and diseases play an important role in the development of calcium metabolism of cardiomyocytes is currently one of the focuses in medical research. The change of intracellular free calcium ([Ca ~ (2 +)] i) in living cells by fluorescence spectroscopy is a new technology that has emerged in recent years. Up to now, the intracellular [Ca ~ (2 +)] i in isolated adult rat myocardial cells has not been reported by the above method. The purpose of this experiment is to establish this method (Fura-2 is