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建立了一种简易、快速测定硫酸链霉素的流动注射化学发光法.本法基于在强碱性介质中,硫酸链霉素能增强铁氰化钾氧化鲁米诺产生化学发光,加入亚铁氰化钾能大大降低这一体系的背景发光强度.在优化的实验条件下该法测定硫酸链霉素的线性范围为5～1000μg/mL,检出限(3σ)为0 6μg/mL.对100μg/mL的硫酸链霉素进行11次平行测定,其RSD为2 2%.将本法用于硫酸链霉素制剂及合成样品的分析,结果令人满意.
A simple and rapid flow-injection chemiluminescence method was developed for the determination of streptomycin sulfate.The method was based on the chemiluminescence of luminol oxidation of luminol by streptomycin sulfate in strong alkaline medium, adding ferrous Potassium cyanide can greatly reduce the background luminous intensity of this system.Under the optimized experimental conditions, the linear range of this method is 5 ~ 1000μg / mL and the detection limit (3σ) is 0.6μg / mL. 100 μg / mL of streptomycin sulfate for 11 replicates with a RSD of 22% The method was satisfactory for the analysis of streptomycin sulfate formulations and synthetic samples.