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以日本进境的冻鳕鱼体内分离出的异尖科线虫为研究对象,用保守引物PCR扩增其核糖体DNA(rDNA)的内转录间隔区(ITS)和5.8S序列,并进行克隆、转化和序列分析,对样品进行分子鉴定。结果获得2个异尖科线虫样品的ITS及5.8 S rDNA序列,总长为991 bp,样品间序列相似性为99.0%。将序列与GenBank公布灰海鳗对盲囊线虫(Contracaecum muraenesoxi)的相关序列进行比较分析,结果显示与C.muraenesoxi的ITS1、5.8S和ITS2序列相似性高,分别98.0%～98.4%、100%和97.0%～98.0%,表明冻鳕鱼中分离的异尖科线虫属于C.muraenesoxi。
In the present study, Ichthyocene nematodes isolated from Japanese frozen cod were used to amplify ITS and 5.8S of ribosomal DNA (rDNA) by PCR using conservative primers and cloned and transformed And sequence analysis, the sample molecular identification. Results ITS and 5.8 S rDNA sequences were obtained from two C. elegans samples with a total length of 991 bp and a similarity of 99.0% among the samples. The results of comparative analysis of the sequences with GenBank published in Gray moray with Contracaecum muraenesoxi showed that the sequences were highly homologous to ITS1, 5.8S and ITS2 sequences of C.muraenesoxi, 98.0% -98.4%, 100% And 97.0% ~ 98.0%, respectively, indicating that the isolates of C. elegans belong to C. muraenesoxi.