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本实验使用ＢＤＦ１雌性小鼠７５只，分动情周期和妊娠初期两组。免疫组织化学ＬＳＡＢ法对小鼠子宫内膜中细胞外基质（ＥＣＭ），包括Ｉ、ＩＶ和Ｖ型胶原蛋白（ＣＬ），层粘连蛋白（ＬＮ）和纤维粘连蛋白（ＦＮ）进行染色，目的为探讨胚泡着床的微环境。结果ＥＣＭ在动情周期变化不明显，而妊娠第４～６天ＬＮ、ＦＮ和Ｖ型ＣＬ增多，Ｉ型和ＩＶ型ＣＬ减少；妊娠７、８天ＬＮ、ＦＮ和Ｖ型ＣＬ减少，Ｉ型和ＩＶ型ＣＬ增多。提示ＥＣＭ中的ＬＮ、ＦＮ和Ｖ型ＣＬ可能是利于胚泡着床的成分，而Ｉ型和ＩＶ型ＣＬ可能是阻止胚泡着床的成分。认为胚泡着床必须在子宫ＥＣＭ构成的有利微环境中才能顺利进行。
In this experiment, 75 BDF1 female mice were used, divided into estrous cycle and early pregnancy. Immunohistochemistry LSAB method was used to stain extracellular matrix (ECM), including type I, IV and type V collagen (CL), laminin (LN) and fibronectin (FN) in mouse endometrium for the purpose of To investigate the microenvironment of blastocyst implantation. Results The changes of ECM in estrous cycle were not obvious, but LN, FN and V-CL increased, CL of type I and IV decreased on the 4th to 6th day of gestation. The LN of CL decreased at 7 and 8 days, Type IV CL increased. It is suggested that LN, FN and V CL in ECM may be the components that facilitate the implantation of blastocysts. CLs I and IV may be the components that prevent the implantation of blastocysts. It is thought that implantation of the blastocyst must proceed smoothly in the favorable microenvironment formed by the uterine ECM.