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为探讨克服ＲＴ－ＰＣＲ法检测恶性肿瘤微转移时出现假阳性的方法，作者运用该法检测了４０例非小细胞肺癌和２０例食管癌患者病理证实有转移的淋巴结中角蛋白１９（ＣＫ１９）基因的表达，同时还分别运用套式ＲＴ－ＰＣＥ或ＲＴ－ＰＣＲ法检测了１０例良性肺和食管肿瘤患者正常淋巴结中ＣＫ１９基因的表达。结果发现设立分别以未经逆转录的ＲＮＡ和基因组ＤＮＡ为模板的对照可观察到是否存在ＣＫ１９假基因的干扰所造成的假阳性，而在逆转录前用脱氧核糖核酸酶（ＤＮａｓｅ）消化ＲＮＡ可消除这种假阳性。运用套式ＲＴ－ＰＣＥ法测定ＤＮａｓｅ消化后的正常淋巴结ＲＮＡ可发现ＣＫ１９基因非法转录所致假阳性，调整方法灵敏度，使之刚好能区分正常和转移淋巴结是解决此类假阳性的合适办法。
In order to explore the method to overcome the false-positive detection of micrometastasis in malignant tumors by RT-PCR, the authors used this method to detect keratin 19 (CK19) in lymph node metastasis confirmed by pathology in 40 patients with non-small cell lung cancer and 20 patients with esophageal cancer. Gene expression was also measured using nested RT-PCR or RT-PCR, respectively, in 10 cases of benign lung and esophageal cancer patients with normal lymph node CK19 gene expression. As a result, it was found that the establishment of a control that uses RNA and genomic DNA as a template without reverse transcription, respectively, can detect the presence of false positives caused by the interference of the CK19 pseudogene, and the digestion of RNA with DNAase (DNase) before reverse transcription can be performed. Eliminate this false positive. The use of nested RT-PCE method to determine normal lymph node RNA after DNase digestion can be found false positive CK19 gene transcription, adjust the sensitivity of the method, so that it can just distinguish between normal and metastatic lymph nodes is a suitable solution to such false positives.