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【目的】观察伯氏嗜木螨Caloglyphus berlesei生活史各发育阶段外部形态和超微形态特征。【方法】采集中华地鳖Eupolyphaga sinensis培养床上的培养料,分离伯氏嗜木螨。将分离获得的伯氏嗜木螨双蒸水洗涤后,一部分用于制备常规玻片标本,光镜下直接观察;另一部分用2.5%戊二醛溶液固定,70%酒精再次洗涤,临界点干燥,置于扫描电镜下观察不同发育阶段(包括卵、幼螨、若螨及成螨)的超微形态特征。【结果】电镜下伯氏嗜木螨各发育阶段的足、刚毛和外生殖器及其附属结构的形态均清晰可辨。幼螨足3对,足上无叶状刚毛,基节干发达;若螨足4对,出现第4背毛,生殖区发育不全;休眠体足爪和前跗节发达,出现叶状毛、胫节毛及膝节毛等结构,生殖板骨化明显,其两侧有吸盘和刚毛各1对。吸盘板上共有4对成对的吸盘、1个单吸盘和2对类圆形微凸。成螨生殖感觉器骨化且呈心形,雄雌成螨生殖感觉器的刚毛数量上有明显差异。【结论】对伯氏嗜木螨形态和超微形态特征的观察有助于对其进一步科学分类和进行生活史研究,并可为控制伯氏嗜木螨及其引起的过敏性疾病提供参考。
【Objective】 To observe the morphological and ultrastructural features of Caloglyphus berlesei at various developmental stages. 【Method】 The culture materials of Eupolyphaga sinensis were collected from the bed of Sophora albiflorus and the mites were isolated. The isolated Parrillae mites double distilled water after washing, a portion for the preparation of conventional slide specimens, direct observation under light microscope; the other part was fixed with 2.5% glutaraldehyde solution, 70% alcohol was washed again, the critical point of drying , Under the scanning electron microscope to observe the different developmental stages (including eggs, larvae, nymphs and adult mites) ultrastructural features. 【Result】 The morphological changes of foot, bristles, genitalia and their ancillary structures in each developmental stage of M. parvum under electron microscope were clearly discernible. There were 3 pairs of larvae on the foot, no leaf bristles on the foot, and the stems developed dryly. If the mite foot was 4 pairs, the 4th dorsal hair appeared and the reproductive zone was hypoplasia. Tibia hair and knee hair and other structures, genital board ossification obvious, on both sides of the sucker and seta each one pair. Suction plate on the board a total of 4 pairs of sucker, a single sucker and two pairs of round convex. Adult mite reproductive sensory ossification and heart-shaped, male and female adult mites reproductive sensory number of bristles there are significant differences. 【Conclusion】 Observations on morphological and ultrastructural features of Murabus granulosus contribute to further scientific classification and life history studies, and provide references for the control of Acremonium spp. And its allergic diseases.