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目的:探讨蓝狐胆汁对D-Glan肝损伤小鼠模型的保护作用。方法:取昆明种小鼠30只,随机分为5组,即空白对照组(空白组)、D-氨基半乳糖(D-Galn)急性肝损伤模型组(造模组)、阳性药葵花护肝(0.4 g/kg)组(阳性组)、蓝狐胆粉高剂量组(10 g/kg)组(高剂量组),蓝狐胆粉低剂量(5 g/kg)组(低剂量组),每组6只;灌胃给药,1次/d,连续10 d,空白对照组予同体积蒸馏水。末次给药前24 h,小鼠腹腔注射D-Galn 500 mg/kg,末次给药前15 h将动物禁食,末次给药后1 h将动物处死,取血清,测定血清中总胆固醇(T-CHO)浓度、谷丙转氨酶(ALT)和谷草转氨酶(AST)的活性;取肝脏,测定肝脏中的丙二醛(MDA)含量,并作病理切片。结果:与模型组比较,蓝狐胆粉高剂量、低剂量组均能显著降低血清ALT、AST、T-CHO及肝脏MDA的含量(P<0.05),表明蓝狐胆汁具有保护D-Galn造成肝损伤的作用。结论:蓝狐胆汁对D-Galn造成肝损伤小鼠模型具有保护作用。
Objective: To investigate the protective effect of blue fox bile on D-Glan liver injury in mice. Methods: Thirty Kunming mice were randomly divided into five groups: control group (blank group), D-Galn acute liver injury model group (model group), positive drug sunflower (0.4 g / kg) group (positive group), high dose group of blue gall bladder powder (10 g / kg), low dose group of blue gall gall powder ), Each group of 6; gavage administration, 1 time / d, continuous 10 d, blank control group to the same volume of distilled water. 24 h before the last administration, the mice were intraperitoneally injected with D-Galn 500 mg / kg, and the animals were fasted 15 h before the last administration. The animals were sacrificed at 1 h after the last administration, serum was taken and the total cholesterol (T (CHO), glutamic-pyruvic transaminase (ALT) and aspartate aminotransferase (AST) were measured. The liver was taken and the content of malondialdehyde (MDA) in the liver was measured. Results: Compared with the model group, the concentrations of ALT, AST, T-CHO and MDA in the liver of high-dose and low-dose group were significantly decreased (P <0.05), indicating that the blue fox bile had protective effect on D-Galn The role of liver damage. Conclusion: Blue fox bile has a protective effect on D-Galn induced liver injury in mice.