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通过虾夷马粪海胆胚胎暴露实验,研究了常见石油烃零号柴油对海胆胚胎发育时间以及发育时期内海胆胚胎的SOD、CAT、GPx及谷胱甘肽转移酶(GST)四种酶活性的影响。结果表明,不同浓度的零号柴油水溶组分对海胆胚胎发育的各个时期具有不同程度的延迟效应,低浓度对海胆胚胎中的四种酶表现出不同程度的诱导效应;四种酶活性的变化程度比较,对GPx和GST酶活力的影响要明显大于对CAT和SOD的影响;随着浓度的不断增大,四种酶活性受到不同程度的抑制作用,高浓度对酶活性的抑制作用尤为显著;囊胚期对影响最为敏感,可作为生物标志物。
Through the experiments on the embryo exposure of horseradish saprophyla in seaweed, the effects of common petroleum hydrocarbon zero diesel on the sea urchin embryonic developmental time and the activities of SOD, CAT, GPx and glutathione transferase (GST) in sea urchin embryos influences. The results showed that different concentrations of No.0 diesel diesel water soluble components have different degrees of delay effects on the developmental stages of sea urchin, low concentrations of four kinds of enzymes in sea urchin embryos showed different degrees of induction effect; four changes in enzyme activity The effect of GPx and GST on the activity of CAT and SOD was significantly greater than that on CAT and SOD. With the increase of concentration, the activities of four enzymes were inhibited to some extent, and the inhibitory effect of high concentration on enzyme activity was especially significant The blastocyst stage is the most sensitive and can be used as a biomarker.