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目的:探讨藤梨根提取物对人胃癌BGC-823细胞凋亡的影响及其调控机制。方法:体外培养对数生长期胃癌BGC-823细胞,采用不同浓度的藤梨根提取物作用于人胃癌BGC-823细胞,流式细胞仪检测细胞凋亡,采用免疫组织化学方法检测Bax蛋白表达。结果:藤梨根作用于BGC-823,在8~32mg·mL-1时呈不同程度的促进凋亡;藤梨根提取物大、中、小剂量组对人胃癌BGC-823细胞中Bax表达水平上调。结论:藤梨根提取物对人胃癌BGC-823细胞的增殖具有明显抑制作用,在实验浓度范围内呈浓度依赖性促进细胞凋亡,这可能与激活Bax蛋白表达有关。
Objective: To investigate the effects of Tengli root extract on the apoptosis of human gastric cancer cell line BGC-823 and its regulatory mechanism. Methods: The gastric cancer BGC-823 cells were cultured in vitro. The gastric cancer BGC-823 cells were treated with different concentrations of rattan root extract. The apoptosis of BGC-823 cells was detected by flow cytometry. The expression of Bax protein was detected by immunohistochemistry . Results: The effect of Tengligen on BGC-823 cells promoted apoptosis at different levels of 8 ~ 32mg · mL-1. Bax expression in human gastric cancer cell line BGC-823 Level up. CONCLUSION: The extract of Tengligen can significantly inhibit the proliferation of human gastric cancer cell line BGC-823 and promote apoptosis in a concentration-dependent manner in a concentration-dependent manner, which may be related to the activation of Bax protein expression.