论文部分内容阅读
BACKGROUND: The inhibitory effect of antisense oligo- deoxynucleotide (asODN) on the replication and expres- sion of hepatitis C virus (HCV) in vitro is not well elucida- ted. This study was to assess the effect of asODN on HCV in cholangiocarcinoma. METHODS: The QBC939 cells transfected by a recombi- nant HCV containing HCV core gene cloned in vector of PBK-CMV (PBK-HCVC ) were treated by 14-mers phos- phorothioate ODN complementary to the HCV core ge- nomic region. The variation of HCVmRNA level was de- tected by RT-PCR. Moreover, the inhibitory effect of asODN was observed in nude mice. RESULTS: HCVmRNA was detected in transfected-QBC- 939 cells. The 14-mers complementary phosphorothioate ODN showed effective inhibition on HCVmRNA and un- expression HCVmRNA at 6 μmol/L. The tumorigenicity of the transfected-QBC939 cells incubated with asODN in nude mice was greatly inhibited. CONCLUSION: The results suggest a potential therapy of asODN for HCV infected cholangiocarcinoma.
BACKGROUND: The inhibitory effect of antisense oligo-deoxynucleotide (asODN) on the replication and expres- sion of hepatitis C virus (HCV) in vitro is not well elucidated. This study was to assess the effect of asODN on HCV in cholangiocarcinoma. METHODS: The QBC939 cells transfected by a recombi- nant HCV containing HCV core gene cloned in vector of PBK-CMV (PBK-HCVC) were treated by 14-mers phos- phorothioate ODN complementary to the HCV core geonomic region. The variation of HCV RNA levels was de- tected by RT-PCR. Moreover, the inhibitory effect of asODN was observed in nude mice. RESULTS: HCV mRNA was detected in transfected-QBC- 939 cells. The 14-mers complementary phosphorothioate ODN showed effective inhibition on HCV mRNA The tumorigenicity of the transfected-QBC939 cells incubated with asODN in nude mice was greatly inhibited. CONCLUSION: The results suggest a potential therapy of as ODN for HCV infected cholangiocarcinoma.