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应用HRP(horseradish Peroxidase)逆行追踪标记,研究大白鼠中脑导水管周围灰质(Periaqueductal gray PAG)尾侧半背外侧区和背侧区的传入投射。将HRP注入PAG尾侧半背外侧区:①大脑皮层出现大量的标记细胞,主要分布在额皮层区、后肢皮层区和顶皮层区,其次是前肢皮层区和嗅周皮层。②基底前脑标记细胞少且分散,仅在终纹床核、无名质和杏仁内侧核内出现少量的标记细胞。③下丘脑主要出现在下丘脑腹内侧核和背侧乳头体前核,其次是视前内侧区、下丘脑前外侧核和灰结节。①底丘脑的未定带出现了大量的标记细胞;后丘脑的标记细胞主要分布于内侧膝状核。将HRP注入到PAG尾侧半背侧区:①标记细胞在大脑皮层内集中分布于额皮层区。②基底前脑仅在终纹床核内出现一定数量的标记细胞.③下丘脑的标记细胞集中分布于下丘脑前外侧核、下丘脑前区、下丘脑腹内侧核和背侧乳头体前核,其次是视前内侧区和下丘脑背内侧核。④底丘脑的未定带出现大量的标记细胞。⑤上丘脑的标记细胞主要位于内侧缰核和前顶盖前核。⑥后丘脑内的内侧膝状核亦出现大量的标记细胞。
Using HRP (horseradish peroxidase) retrograde tracing labeling, we investigated the afferent projection in the caudal lateral and dorsal flank region of periaqueductal gray PAG in rats. The HRP was injected into the dorsal lateral dorsallateral region of PAG: (1) A large number of labeled cells appeared in the cerebral cortex, mainly in frontal cortex, hindlimb cortex and top cortex, followed by forelimb cortex and olfactory mucosa. ② basal forebrain marker cells less and scattered, only in the terminal bed nucleus, immature and almond medial nucleus a small amount of labeled cells. Hypothalamus mainly in the hypothalamic ventromedial nucleus and dorsal papillary body anterior nucleus, followed by the preoptic medial area, the hypothalamic anterolateral nucleus and gray nodules. ① hypothalamic undetermined zone appeared a large number of labeled cells; posterior thalamus marked cells are mainly distributed in medial geniculate nucleus. HRP into the tail side of the dorsal pAG: ① marker cells in the cerebral cortex concentrated in the frontal cortex. ②The basal forebrain only appeared a certain number of labeled cells in the nucleus of the terminal cochlea.③The labeled cells in the hypothalamus were concentrated in the hypothalamic anterior lateral nucleus, hypothalamic anterior region, hypothalamic ventrolateral medulla and dorsal papillary body anterior nucleus , Followed by the preoptic medial area and the hypothalamic dorsomedial nucleus. ④ hypothalamic undetermined zone with a large number of labeled cells. ⑤ labeled cells in the hypothalamus is mainly located in the medial habenular nucleus and the anterior canopy. ⑥ hypothalamic medial geniculate nucleus also appeared a large number of labeled cells.