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目的检测宫颈癌患者鳞癌组织中CXCR4的表达,观察其与宫颈鳞癌各种临床病理参数的关系,从而为探讨CXCR4与宫颈癌侵袭性的机制研究提供一定的实验依据。方法收集我院妇产科收治的145例宫颈癌手术治疗患者临床病理资料及切除标本,采用免疫组织化学SP法检测标本中CXCR4的表达情况,并通过Transwell实验了解CXCR4过表达的细胞迁移情况。结果不同FIGO病理分期、不同组织分化程度及不同淋巴结转移情况的患者之间,CXCR4表达的阳性率差异有统计学意义(P<0.05),FIGO分期为Ⅱ期及Ⅲ期者阳性率较高,组织分化程度越低者阳性率越高,发生淋巴结转移者CXCR4表达的阳性率高于未转移者。宫颈鳞癌细胞迁移实验结果显示,除CXCR4过表达3 h和6 h两个时点外,其余各时间点宫颈鳞癌细胞过表达CXCR4组与对照组相比较,平均迁移细胞数的差异均具有统计学意义(P<0.05)。结论宫颈鳞癌组织中CXCR4高表达可能在宫颈鳞癌的侵袭、转移中起重要作用,对细胞侵袭和转移能力有促进作用。
Objective To detect the expression of CXCR4 in squamous cell carcinoma of cervical cancer and observe its relationship with clinicopathological parameters of cervical squamous cell carcinoma so as to provide some experimental evidence for exploring the mechanism of CXCR4 and invasiveness of cervical carcinoma. Methods The clinicopathological data and excision specimens of 145 cases of cervical cancer treated by gynecology and obstetrics in our hospital were collected. The expression of CXCR4 in specimens was detected by immunohistochemical SP method. The migration of CXCR4 over-expressed cells was detected by Transwell assay. Results The positive rate of CXCR4 expression was significantly different between patients with different FIGO pathological stages, differentiation degree of different tissues and different lymph node metastasis (P <0.05), the positive rate of FIGO stage Ⅱ and Ⅲ was higher, The higher the positive rate was, the lower the degree of differentiation was. The positive rate of CXCR4 expression in lymph node metastasis was higher than that in non-metastasis. The results of cervical squamous cell carcinoma migration showed that except the CXCR4 overexpression 3 h and 6 h two time points, the other time points of cervical squamous cell carcinoma CXCR4 overexpression compared with the control group, the average number of cells were migrated with Statistical significance (P <0.05). Conclusion The high expression of CXCR4 in cervical squamous cell carcinoma may play an important role in the invasion and metastasis of cervical squamous cell carcinoma and promote the invasion and metastasis of cervical squamous cell carcinoma.