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为了全面了解经济林木离体培养现状和找出离体培养的规律 ,概述了经济林木胚胎培养、器官培养、愈伤组织培养、细胞悬浮培养、原生质体培养、原生质体融合和人工种子等方面的研究现状 .到目前为止 ,已离体培养成功的经济林木种类有 5 0余种 ,其中 ,原生质体培养成功的种类不足 10种 .并指出 ,经济林木愈伤组织再生体系的建立是细胞培养、原生质体培养和原生质体融合的基础 .原生质体培养体系的建立对各种遗传操作和利用生物技术培育新品种具有重要意义 .
In order to fully understand the status quo of economic forestry in vitro culture and to find out the rules of in vitro culture, the economic aspects of embryo culture, organ culture, callus culture, cell suspension culture, protoplast culture, protoplast fusion and artificial seeds were summarized So far, more than 50 species of economic trees have been successfully cultured in vitro, of which less than 10 species were successfully cultivated.It also pointed out that the establishment of economic tree callus regeneration system is the key to cell culture, The basis of protoplast culture and protoplast fusion.The establishment of protoplast culture system is of great significance to all kinds of genetic manipulation and the use of biotechnology to cultivate new varieties.