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一、材料和方法 1.材料:本试验采用四唑染色法测定种子生活力,普通发芽试验测定种子的发芽率。扦取水稻样品有:浙辐802、钱青、圭福3号、791163、威优35、威优63、汕优63、军莲早8个品种。 2.方法:先从每个品种中取出200粒种子,将其脱壳后,在室温下清水浸6小时,然后沿胚对切呈半粒冲洗,再浸于0.25%红四唑的溶液中,在30℃恒温下放置2小时(黑暗处),染色测定种子的生活力。再从每个品种中取400粒种子,测定其发芽率,比较两组结果。二、试验结果水稻种子生活力(x)与发芽率(y)试验结果
First, materials and methods 1. Materials: The test using tetrazolium staining method to determine seed viability, the average germination test seed germination rate. Stems taken rice samples are: Zhejiang spokes 802, Qian Qing, Guifu 3, 791163, Wei You 35, Weiyou 63, Shanyou 63, Junlian as early as 8 varieties. 2. Methods: Firstly, 200 seeds were taken from each variety. After shelling, they were soaked in clear water for 6 hours at room temperature, then washed halfway along the embryos and then immersed in 0.25% red tetrazolium solution , Left standing at a constant temperature of 30 ° C for 2 hours (dark place), and the viability of the seed was measured by staining. 400 seeds were taken from each variety, and the germination rate was measured. The results of two groups were compared. Second, the test results Rice seed viability (x) and germination rate (y) test results