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为了解67份美国材料的遗传多样性及其醇溶蛋白亚基对品质性状的影响,利用酸性聚丙烯酰胺凝胶电泳(APAGE)技术进行醇溶蛋白谱带分析,测定了面团流变学特性及理化品质。结果表明,在67份美国材料中共分离出1332条谱带,49种不同迁移率类型的谱带,大部分谱带具有多态性。单个材料谱带总数变异幅度为13~28。谱带数在α、β、γ、ω4个区的分布存在较大差异。没有发现电泳谱带完全相同的材料。GS值变异范围0.54~0.90,平均值为0.731。在GS=0.607水平上,聚类分析将这67份材料分为6类。49条不同迁移率的谱带中有17条谱带与36项品质性状的相关性达到显著或极显著差异。6条谱带(迁移率为49.6、56.2、56.7、62.2、79.4、86.8)与湿面筋含量、蛋白质含量和沉淀值呈正相关,而迁移率为60.5的谱带与之呈负相关。11条谱带(迁移率为26.5、42.0、49.6、52.5、56.2、56.7、62.2、64.1、72.0、79.4、86.8)与面团稳定时间、面团形成时间、延伸面积等面团流变学特征呈正相关,而迁移率为34.4、47.5、49.0、60.5、69.4、85.4的6条谱带则与之呈负相关。说明供试材料间存在着丰富的遗传多样性以及与优质品质相关的谱带,为进一步利用这67份种质资源和优质小麦品种的选育提供了理论依据。
In order to understand the genetic diversity of 67 American materials and the effects of prolamine subunits on the quality traits, the prolamin bands were analyzed by acidic polyacrylamide gel electrophoresis (APAGE), and the rheological properties of dough And physical and chemical quality. The results showed that 1332 bands and 49 bands of different mobility types were isolated in 67 U.S. materials, most of which were polymorphic. The variation range of the total number of single material bands is 13 ~ 28. There are large differences in the distribution of band numbers in the four regions of α, β, γ and ω. No identical bands were found. The variation of GS value ranged from 0.54 to 0.90 with an average value of 0.731. At the level of GS = 0.607, the cluster analysis divided the 67 materials into six categories. Correlation of 17 bands and 36 quality traits in 49 bands with different mobility reached significant or extremely significant differences. The six bands (mobility 49.6, 56.2, 56.7, 62.2, 79.4, 86.8) were positively correlated with wet gluten content, protein content and sedimentation values, whereas the bands with a mobility of 60.5 were negatively correlated. The 11 bands (mobility 26.5,42.0,49.6,52.5,56.2,56.7,62.2,64.1,72.0,79.4,86.8) were positively correlated with the dough rheological characteristics such as dough stability time, dough forming time and extending area, The six bands with a mobility of 34.4, 47.5, 49.0, 60.5, 69.4 and 85.4 were negatively correlated with them. The results showed that there were rich genetic diversity and high quality-related bands among the tested materials, which provided a theoretical basis for further utilization of the 67 germplasm resources and breeding of high-quality wheat varieties.