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本实验用家兔18只,动物麻醉后分离双侧膈神经,用30Hz的方波(0.2ms,10V)持续刺激膈神经致伤复制膈肌疲劳,分别测量伤前及伤后30min,60min.90min,120min的跨膈压及膈肌诱发电位(DEP)的变化.结果表明;刺激30min后最大跨膈压和诱发电位幅度明显降低,诱发电位潜伏期显著延长(n=7).膈肌疲劳后,静脉注射新斯的明0.05mg·kg~(-1),可迅速提高跨膈压和DEP幅度及缩短DEP潜伏期(n=6),而生理盐水组变化不明显(n=5).提示:(1)DEP可以作为评价膈肌疲劳的一项指标;(2)新斯的明有抗膈肌疲劳作用.
In this experiment, 18 rabbits were anesthetized and the bilateral phrenic nerve was isolated. The diaphragmatic fatigue was replicated with 30Hz square wave (0.2ms, 10V) continuously. The pre-injury and post-injury 30min, 60min 90min , Diastolic pressure (120min) and diastolic pressure (DEP) of diaphragmatic muscle.The results showed that the maximal transdiaphragmatic pressure and evoked potential amplitude were significantly reduced and the evoked potential latency was significantly prolonged (n = 7) after 30min stimulation.After the diaphragm was exhausted, Neostigmine 0.05mg · kg ~ (-1) can rapidly increase diastolic pressure and DEP amplitude and shorten the latent period of DEP (n = 6), while the saline group did not change significantly (n = 5) ) DEP can be used as an indicator of diaphragmatic fatigue; (2) Neostigmine has anti-diaphragmatic fatigue effects.