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提出了一种新的高灵敏测定抗坏血酸的荧光分析法.抗坏血酸与Cu2+在pH为7.40的磷酸盐缓冲溶液中生成羟基自由基,然后羟基自由基与苯甲酸反应形成荧光物质.建立了测定抗坏血酸的适合条件,测定抗坏血酸的线性范围为(0.0128～1.92)×10-6g/mL,方法的检测限是7.75×10-9g/mL.对于8.56×10-7g/mL的抗坏血酸测定6次,其相对标准偏差为1.6%.
A new fluorimetric method for the determination of ascorbic acid was proposed.Ascorbic acid and Cu2 + generated hydroxyl radicals in a phosphate buffer solution with a pH value of 7.40 and then reacted with benzoic acid to form a fluorescent substance.A method was developed for the determination of ascorbic acid The suitable conditions for the determination of ascorbic acid in the linear range of (0.0128 ~ 1.92) × 10-6g / mL, the detection limit of the method is 7.75 × 10-9g / mL for 8.56 × 10-7g / mL ascorbic acid determination 6 times, the relative The standard deviation is 1.6%.