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以苹果火疫病菌 (Erwiniaamylovora)的Harpin蛋白基因和马铃薯 prp1 1基因启动子为主要元件 ,探索了以利用病原侵染诱导植物过敏性反应为目标的抗病基因工程新策略 .通过构建Harpin蛋白基因的 3个植物表达载体和遗传转化 ,获得68个转基因马铃薯植株 .Southern ,Northern和Westernblot分析证明 ,Harpin蛋白基因实现了在转基因植株中的插入、转录和蛋白表达 ,用Phytophthorainfestans复合生理小种测定表明 ,Harpin蛋白在转基因植株中的组成型表达和病原侵染诱导表达均能降低病斑的扩展速率 ;在病原侵染诱导Harpin蛋白基因表达的转基因植株中 ,发现有 2个植株共 3 0个接种叶片上无菌丝形成 ,病斑局限于接种点内 ,表明过敏性反应的遗传操作在植物抗真菌病基因工程中具有广阔的应用前景 .
Taking Harpin protein gene and promoter of potato prp1 1 gene of Erwinia amylovora as the main components, a new strategy of disease resistance gene engineering targeting to induce plant hypersensitive reaction by pathogen infection was explored. By constructing Harpin protein gene Of the three plant expression vectors and genetic transformation to obtain 68 transgenic potato plants Southern, Northern and Western blot analysis showed that the Harpin protein gene in transgenic plants to achieve insertion, transcription and protein expression, with Phytophthorainfestans compound physiological race showed that , Harpin protein expression in transgenic plants and pathogenic infection induced expression can reduce the spread of disease spots; in the pathogen infection induced Harpin protein gene expression in transgenic plants, found that a total of 2 plants were 30 vaccination The formation of asexual leaves on the leaves, the lesion confined to the inoculation point, indicating that the genetic manipulation of allergic reactions in plant antifungal gene engineering has broad application prospects.