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脓毒症是各种微生物及免疫原性物质引起的全身炎症反应和宿主自身免疫性损伤,其本质是大量炎症因子和炎症介质失控性释放导致的宿主自身免疫性损伤(autoimmunity injury)。脓毒症是当今医学所面临的主要挑战之一,也是儿科疾病的死亡原因之一,进一步发展可引起严重脓毒症(severe sepsis)、脓毒症休克(septic shock)和多器官功能障碍综合征(Multiple Organ Dysfunction Syndrome MODS)甚至死亡。脓毒症的发病机制尚不十分明确,可能的发病机制包括:炎症因子风暴、凝血系统紊乱和微循环、线粒体功能障碍等。线粒体功能障碍在脓毒症,尤其是脓毒症休克患者的病情发展中起了很重要的作用。但在脓毒症恢复过程中,线粒体机制尚不明确。线粒体生物修复(Mitochondrial biogenesis)是维持及恢复线粒体结构与功能的一种细胞程序,在脓毒症恢复中起着重要的作用。在某些因素(尚未明确)的启动下,脓毒症器官线粒体再生修复的相关基因表达增加,可增加线粒体DNA的复制与转录,使线粒体相关的功能与结构蛋白表达增加,恢复线粒体的功能。肾脏是机体重要器官,是脓毒症时易损器官。本课题对脓毒症大鼠肾脏的功能状态,肾脏线粒体功能状态、超微形态结构及线粒体生物修复基因表达进行研究,探讨脓毒症肾脏功能损伤与线粒体损伤及修复间的关系,进一步了解脓毒症的病理生理机制。
Sepsis is a systemic inflammatory response and host autoimmune damage caused by various microbes and immunogenic substances. The essence of sepsis is autoimmunity injury caused by a large number of inflammatory factors and uncontrolled release of inflammatory mediators. Sepsis is one of the major challenges in medical science today and one of the causes of pediatric morbidity and further development can cause severe sepsis, septic shock and multiple organ dysfunction syndrome Multiple Organ Dysfunction Syndrome (MODS) even died. The pathogenesis of sepsis is not yet clear, possible pathogenesis include: inflammation of the storm, coagulation disorders and microcirculation, mitochondrial dysfunction. Mitochondrial dysfunction plays a very important role in the progression of sepsis, especially in patients with septic shock. However, mitochondrial mechanisms remain unclear during sepsis recovery. Mitochondrial biogenesis is a cellular process that maintains and restores mitochondrial structure and function and plays an important role in the recovery of sepsis. In some factors (not yet clear) to start, sepsis organ mitochondrial regeneration and repair of genes related to increased expression can increase mitochondrial DNA replication and transcription, mitochondria-related functions and structural protein expression increased recovery of mitochondrial function. The kidney is a vital organ of the body and is a vulnerable organ in sepsis. The topic of renal function in rats with sepsis, renal mitochondrial functional status, ultrastructure and mitochondrial biogenesis repair gene expression studies to explore the relationship between sepsis renal dysfunction and mitochondrial damage and repair, to further understand the pus Pathophysiology of the disease.