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1引言本研究尝试一种新的填料羟基磷灰石（HAP）用梯度洗脱方法分离纯化基因重组人白细胞介素．2（II，2）。研究结果表明，用本实验方法，大量上样提纯后的兀2在纯度、活性等方面均满足要束，效果良好。z实验部分2．1试剂及仪器羟基磷灰石填料（中国科学院化工冶金研究所），
1 Introduction In this study, we tried a new packing of hydroxyapatite (HAP) gradient elution method for the isolation and purification of recombinant human interleukin. 2 (II, 2). The results show that, with this experimental method, a large number of samples after purification Wu 2 in the purity, activity, etc. are to meet the beam, the effect is good. z Experimental 2.1 Reagents and Equipment Hydroxyapatite Filler (Institute of Chemical Metallurgy, Chinese Academy of Sciences),