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采用水培方法,研究了盐碱与Spd处理对两品种番茄(中杂9号和金棚朝冠)幼苗氮代谢及主要矿质元素含量的影响.结果表明:盐碱胁迫下,番茄幼苗干生物量显著减少,植株生长受到抑制;叶片和根系硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)活性及硝态氮(NO_3~--N)、全N、全K、Ca~(2+)、Mg~(2+)含量显著降低,铵态氮(NH_4~+-N)、Na~+含量显著增加;两品种叶片及中杂9号根系谷氨酸脱氢酶(GDH)活性显著升高,金棚朝冠根系GDH活性变化不显著;叶片全P含量显著降低,根系全P含量显著升高(金棚朝冠)或无显著变化(中杂9号).Spd处理通过增强NR、GS、GOGAT活性提高了植株对NH_4~+的同化利用率,有效缓解了盐碱胁迫导致的氮代谢紊乱,进而促进不同器官对P、K、Ca、Mg、Na的吸收、释放或转运,在一定程度上维持了各元素之间的相对平衡,从而增强植株对逆境的适应能力.此外,盐碱对中杂9号的抑制作用及外源Spd对其氮代谢紊乱和营养失衡的缓解作用高于金棚朝冠.
The effects of salinization and Spd treatments on nitrogen metabolism and main mineral elements contents in seedlings of two tomato cultivars (Zhongza 9 and Jinpengchaguan) were studied by using hydroponic culture method.The results showed that under saline-alkali stress, The content of nitrate reductase (NR), glutamine synthetase (GS), glutamic acid synthase (GOGAT) activity and nitrate nitrogen (NO 3 - -N) in leaves and roots were all significantly decreased N, total K, Ca ~ (2 +) and Mg ~ (2+) decreased significantly, NH4 + -N and Na ~ + contents increased significantly. The activity of GDH was significantly increased, while the activity of GDH in root of canopy did not change significantly. The content of total P was significantly decreased, the content of total P in root was significantly increased Miscellaneous 9) .Spd treatment enhanced the assimilation and utilization rate of NH_4 ~ + by enhancing NR, GS and GOGAT activity, effectively relieved the nitrogen metabolism disorder caused by salt-alkali stress, and then promoted the accumulation of P, K, Ca, Mg, Na absorption, release or transport, to some extent to maintain the relative balance between the various elements, thereby enhancing plant adaptability to adversity.In addition, The inhibitory effect of Zhongza 9 and exogenous Spd on nitrogen metabolism disorders and nutritional imbalance of the relief effect is higher than the gold shed towards the crown.