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目的研究携带人β干扰素基因(hIFN-β)的重组腺病毒转染骨髓间充质干细胞(MSCs-hIFN-β)以及其在体外抑制大鼠C6胶质瘤细胞。方法转染的MSCs行PT-PCR检测细胞内hIFN-βmRNA的表达;ELISA法检测培养液上清hIFN-β的分泌情况;MTT法检测细胞活力并绘制转染后MSCs生长曲线,MSCs-hIFN-β和C6胶质瘤细胞体外共培养,检测C6胶质瘤细胞活力和共培养液上清hIFN-β含量。结果 Ad-hIFN-β转染的MSCs分化后有绿色荧光蛋白表达,RT-PCR证实MSCs中有hIFN-βmRNA表达,ELISA测定显示MSCs分泌hIFN-β,而且转染MSCs的增殖能力无改变。体外共培养发现C6胶质瘤细胞的生长被不同程度的抑制,C6胶质瘤细胞培养上清hIFN-β的含量随着MSCs-hIFN-β的密度增加而增加。结论在体外MSCs-hIFN-β能抑制大鼠C6胶质瘤细胞的增殖。
Objective To study the effect of recombinant adenovirus carrying human interferon beta gene (hIFN-β) on bone marrow mesenchymal stem cells (MSCs-hIFN-β) and its inhibition of rat C6 glioma cells in vitro. The expression of hIFN-βmRNA was detected by PT-PCR and the secretion of hIFN-β in the culture supernatant was detected by ELISA. The viability of MSCs was measured by MTT assay and the MSCs-hIFN- β and C6 glioma cells in vitro co-culture, C6 glioma cell viability and co-culture supernatant hIFN-β content. Results Ad-hIFN-βtransfected MSCs differentiated into green fluorescent protein, and RT-PCR confirmed the expression of hIFN-βmRNA in MSCs. ELISA showed that MSCs secreted hIFN-β, and the proliferation ability of MSCs did not change. In vitro co-culture found that C6 glioma cell growth was inhibited to varying degrees, C6 glioma cell culture supernatant hIFN-β content with the MSCs-hIFN-β density increased. Conclusion MSCs-hIFN-β can inhibit the proliferation of rat C6 glioma cells in vitro.