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目的:采用人类口腔微生物芯片(HOMINGS-Human Oral Microbe Identification using Next Generation Sequencing)分析拥有不同患龋状态双胞胎及其母亲龈上菌斑中菌群的结构及微生物多样性。方法:牙菌斑样本取自14对双胞胎、1组三胞胎儿童(年龄3~6岁)和15位母亲,共46份样本,分别提取龈上菌斑细菌总DNA,采用HOMINGS测试分析牙菌斑中菌群结构及微生物的多样性。结果:通过高通量测序共获得2293650条有效序列,使用QIIME软件基于97%的相似性聚类OTUs(operational taxonomic unit),得到20431个OTU,经物种注释获得11个门,116个属和139个种水平的物种。在门水平上,母亲组在拟杆菌门和厚壁菌门的丰度显著高于有龋组和无龋组。在属水平上,有龋组和无龋组比较,有龋组在链球菌属、乳杆菌属、放线菌属、嗜血杆菌的丰度要高于无龋组(P<0.05);母亲组的微生物种类较儿童组多,分别在普氏菌属、坦纳氏菌属等10种微生物的相对丰度高于儿童组(P<0.05﹚。结论:母亲组龈上菌斑微生物多样性及优势微生物属水平上丰度显著高于有龋组和无龋组;链球菌属、乳杆菌属、嗜血杆菌属和放线菌属可能为乳牙早期龋(early childhood caries,ECC)的生物标志物。
OBJECTIVE: To analyze the structure and microbial diversity of the flora in the supraginggoristic plaque of twins with different caries status and their mothers using the HOMINGS-Human Oral Microbe Identification using Next Generation Sequencing. Methods: The plaque samples were obtained from 14 pairs of twins, one group of triplets children (aged 3 to 6 years) and 15 mothers. A total of 46 samples were collected. Total bacterial DNA of the supragingular bacterial plaque was extracted. HOMINGS test Bacterial flora in the plaque and microbial diversity. Results: A total of 2293650 validated sequences were obtained by high-throughput sequencing. QIIME software was used to classify 20431 OTUs based on 97% similarity taxonomic units (OTUs). 11 phylogenetics, 116 genera and 139 A variety of levels of species. At the door level, the abundance of mothers in Bacteroidetes and Firmicutes was significantly higher than that in caries-bearing and caries-free groups. At the genus level, there was a significant increase in the abundance of Streptococcus, Lactobacillus, Actinomyces and Haemophilus in caries-bearing and caries-free groups compared with caries-free and caries-free groups (P <0.05) The number of microorganisms in the group was higher than that in the children. The relative abundance of 10 microorganisms such as Prevotella and Tanneria were higher than those in the children (P <0.05). CONCLUSIONS: The diversity of the supragingival plaque and the abundance of dominant microorganisms in the mother group are significantly higher than those in the caries-bearing group and the caries-free group. Streptococcus, Lactobacillus, Haemophilus and Actinomyces may be the primary teeth Biomarkers of early childhood caries (ECC).