Based on piezoresistive effect, the acceleration sensitivity of multi-walled canbon nanotube (MWNT) films was investigated. A three-point bending technique was presented to measure the piezoresistivity, which used a bending stress applied to the samples while making MWNT films wheeling with a rotational machine. The experimental results showed that the fractional increase in resistance increases linearly versus the increase of centripetal acceleration, and there is a linear relationship between the acceleration and the strain. These shed light on using carbon nanotube films as acceleration sensors for many potential applications. Based on piezoresistive effect, the acceleration sensitivity of multi-walled canbon nanotube (MWNT) films was investigated. A three-point bending technique was presented to measure the piezoresistivity, which used a bending stress applied to the samples while making MWNT films wheeling with a rotational machine. The experimental results showed that the fractional increase in resistance increases linearly versus the increase of centripetal acceleration, and there is a linear relationship between the acceleration and the strain. These shed light on using carbon nanotube films as acceleration sensors for many potential applications .