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目的 :rBPI2 3基因 (BPI60 0bp)克隆与鉴定。方法 :采用RT PCR技术 ,从HL 6 0细胞mRNA中扩增编码BPIN端 199个氨基酸 (rBPI2 3)的基因片段 (BPI60 0bp) ,经酶切后通过连接反应构建重组克隆载体 ,测序鉴定。结果 :RT PCR获得预期的扩增产物—BPI60 0bp基因片段。成功构建PUC18 BPI180 、PUC18 BPI4 2 0 重组克隆载体 ,酶切、酶谱分析与预期结果相符。DNA测序结果与文献报道一致。结论 :PUC18 BPI180 、PUC18 BPI4 2 0 重组克隆载体构建成功 ;BPI60 0bp基因序列与文献报道一致
Objective: Cloning and identification of rBPI2 3 gene (BPI60 0bp). Methods: RT-PCR was used to amplify the 199 bp fragment of BPIN (BPI60 0bp) from the mRNA of HL-60 cells. After digestion, the recombinant cloning vector was constructed and identified by sequencing. Results: RT PCR obtained expected amplification product-BPI60 0bp gene fragment. Successfully constructed PUC18 BPI180, PUC18 BPI420 recombinant cloning vector, digestion, zymogram analysis and the expected results. DNA sequencing results reported in the literature. Conclusion: PUC18 BPI180, PUC18 BPI4 2 0 recombinant cloning vector was constructed successfully; BPI60 0bp gene sequence was reported in the literature