论文部分内容阅读
目的:研究脑舒胶囊对阿尔茨海默病模型大鼠的防治效果及其作用机理。方法:双侧脑室注射Aβ25-35复制阿尔茨海默病大鼠模型;Morris水迷宫观察大鼠学习记忆能力;免疫组织化学法(SABC法)检测大鼠海马Bcl-2、Bax及caspase-3的表达;HE染色光镜观察海马神经元损伤情况。结果:与假手术组比较,模型组大鼠的逃避潜伏期明显延长,跨越虚拟平台的次数及在平台象限停留的时间明显减少;Bcl-2和Bax表达均有所增加,其平均光密度比值(Bcl-2/Bax)明显下降;Caspase-3阳性表达明显增高。与模型组比较,0.72mg/kg和1.44mg/kg脑舒胶囊可缩短大鼠逃避潜伏期,增加大鼠跨越虚拟平台的次数及在平台象限停留的时间;使Bcl-2阳性表达明显所增加,Bax阳性表达明显减少,升高Bcl-2/Bax值;明显降低Caspase-3的阳性表达;光镜观察发现模型大鼠海马神经元排列较紊乱、数量减少、体积变小,胞浆浓缩,胞核固缩深染,结构不清,各剂量的脑舒胶囊均能在一定程度上改善上述神经元损伤。结论:脑舒胶囊可改善阿尔茨海默病大鼠的记忆能力及Aβ25-35所致海马神经元损伤,其原因与其提高抗凋亡基因Bcl-2的表达,抑制促凋亡基因Bax表达和caspase-3表达,进而抑制海马神经元的凋亡有关。
Objective: To study the preventive and therapeutic effects of Naoshu capsule on Alzheimer’s disease model rats and its mechanism. Methods: The rat model of Alzheimer’s disease was duplicated with Aβ25-35 injected into bilateral ventricle. The learning and memory ability of rats was observed by Morris water maze. The expressions of Bcl-2, Bax and caspase-3 in hippocampus were detected by immunohistochemistry (SABC) The expression of hippocampal neurons was observed by HE staining. Results: Compared with the sham-operated group, the escape latency of the model group was significantly prolonged, the number of crossing the virtual platform and the time spent in the platform quadrant decreased significantly; the expression of Bcl-2 and Bax increased, the average optical density ratio Bcl-2 / Bax) was significantly decreased; Caspase-3 positive expression was significantly increased. Compared with the model group, Naoshu Capsules of 0.72mg / kg and 1.44mg / kg can shorten the escape latency of rats, increase the number of rats crossing the virtual platform and stay time in the platform quadrant; make the expression of Bcl-2 positive obviously, The positive expression of Bcl-2 and Bcl-2 / Bax were significantly decreased, the expression of Bcl-2 / Bax was significantly decreased, and the positive expression of Caspase-3 was significantly decreased. The light microscopy showed that the hippocampal neurons were disordered, Nuclear shrinkage dark, unclear structure, the dose of Naoshu Capsule to some extent, can improve the above neuronal injury. Conclusion: Naoshu capsule can improve the memory ability of Alzheimer’s disease rats and damage of hippocampal neurons induced by Aβ25-35, which is related to the increase of the expression of anti-apoptotic gene Bcl-2, the inhibition of the expression of pro-apoptotic gene Bax and caspase-3 expression, thereby inhibiting the apoptosis of hippocampal neurons.