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解决壳聚精的水不溶性，拓宽壳聚精的应用范围。方法：直接用过氧化氢对高分子量、高脱乙酸化度的壳聚糖进行非均相降解、制备了水溶性甲壳低聚糖产品，对产品进行红外光谱结构表征，并用高效毛细管电泳测定了寡糖的聚合度。探讨了温度、过氧化氢浓度对降解所需时间、产品得率及甲壳低聚糖聚合度的影响。结果：选择降解温度 ８０℃～９０℃，过氧化氢浓度 ８％～１０％时，水解时间０．５ ～１．０ ｈ，水溶性产品得率为 ４０％～４５％，聚合度为３～７的寡糖占水解产物的３６％～５０％。结论：该方法制备甲壳低聚糖是可行的。如进一步研究工艺条件，有望进行工业化生产。
Solve the water insoluble shell concentrate, broaden the scope of application of fine shell. Methods: Hydrogen peroxide was used to heterogeneously degrade chitosan with high molecular weight and high degree of deacetylation. Water-soluble chitooligosaccharides were prepared. The products were characterized by FTIR spectroscopy. The products were characterized by high performance capillary electrophoresis Oligosaccharide degree of polymerization. The effects of temperature and hydrogen peroxide concentration on the degradation time, product yield and chitooligosaccharides degree of polymerization were discussed. Results: When the degradation temperature was between 80 ℃ and 90 ℃ and the hydrogen peroxide concentration was between 8% and 10%, the hydrolysis time was 0.5-1.0 h, the yield of water-soluble products was 40% -45% and the degree of polymerization was 3 ~ The oligosaccharide of 7 accounts for 36% to 50% of the hydrolyzate. Conclusion: The preparation of chitooligosaccharides is feasible. Such as further study of process conditions, is expected to carry out industrial production.