In groundwater-dependent ecosystems, groundwater circulation controls the overall water quality and ecosystem dynamics. Groundwater and vegetation across a 30-km groundwater transect linking oasis, desert and river in an extremely arid area were investigated with a series of soil profiles drilled into the unsaturated zone to understand groundwater circulation and its control on groundwater quality and surface vegetation in the extremely arid Lower Tarim River, NW China. Measurements have included water-table depth, water chemistry and water isotopes (2H, 18O, 3H) for 15 water samples, soil moisture and chloride content for 11 soil profiles, and vegetation investigation. Results show that the groundwater in desert zone is characterized by slow recharge rate (pre-modern water), great water-table depth (6.17-9.43 m) and high salinity (15.32-26.50 g/L), while that in oasis (uncultivated land) and riparian zone is characterized by relatively fast recharge rate (modern water), small groundwater-table depth (3.56-8.36 m) and low salinity (1.25-1.95 g/L). Stable isotopes show that secondary evaporation takes place during irrigation in oasis. The vegetation characteristics (coverage, richness, evenness and number of plants) are closely related to soil moisture and water-table depth. Groundwater recharge from irrigation in oasis and from river in riparian zone sustains a better ecosystem than that in the desert area, where lateral and vertical groundwater recharge is limited. The more evapotranspirative enrichment may occur in the vegetated and water-rich riparian zone as compared to desert. This study also demonstrates the effectiveness of environmental tracers in studying ecohydrological processes in arid regions. In groundwater-dependent ecosystems, groundwater and vegetation across a 30-km groundwater transect linking oasis, desert and river in an extremely arid area were investigated with a series of soil profiles drilled into the unsaturated zone to understand groundwater circulation and its control on groundwater quality and surface vegetation in the extremely arid Lower Tarim River, NW China. Measurements include included water-table depth, water chemistry and water isotopes (2H, 18O, 3H) for 15 water samples, Soil moisture and chloride content for 11 soil profiles, and vegetation investigation. Results show that groundwater in desert zone is characterized by slow recharge rate (pre-modern water), great water-table depth (6.17-9.43 m) and high salinity 15.32-26.50 g / L), while that in oasis (uncultivated land) and riparian zone is characterized by relatively fast recharge rate (modern water), small groundwa Stable isotopes show that secondary evaporation takes place during irrigation in oasis. The vegetation characteristics (coverage, richness, evenness and number of plants) are closely related to soil moisture and water-table depth. Groundwater recharge from irrigation in oasis and from river in riparian zone sustains a better ecosystem than that in the desert area, where lateral and vertical groundwater recharge is limited. The more evapotranspirative enrichment may occur in the vegetated and water-rich riparian zone as compared to desert. This study also demonstrates the effectiveness of environmental tracers in studying ecohydrological processes in arid regions.