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目的弱阳离子(WCX)磁珠联合应用基质辅助激光解析电离化飞行时间质谱(MALDI-TOF-MS)技术分析2型糖尿病大血管并发症患者血清蛋白组,筛选差异蛋白峰并建立决策树模型,初步探讨所建立的决策树模型在2型糖尿病大血管并发症早期鉴别诊断中的临床意义。方法 55例2型糖尿病大血管并发症患者,84例2型糖尿病无大血管并发症患者,采用WCX联合MALDI-TOF-MS技术获得其蛋白质谱,分析差异并建立决策树模型,扩大样本量,通过盲法验证诊断模型的可靠性。结果建立了由质荷比为16 062.20、31 883.60和7 986.76差异蛋白峰组成的2型糖尿病大血管并发症决策树诊断模型,扩大样本量盲筛敏感性为96.3%,特异性分别为100.0%。结论应用WCX磁珠联合MALDI-TOF-MS技术可筛选出2型糖尿病大血管并发症患者的差异蛋白峰,建立的决策树模型具有的一定的临床应用前景。
Objective To detect serum proteome in patients with macrovascular complications of type 2 diabetes by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) combined with weak cation (WCX) magnetic beads, screen the differential protein peaks and establish a decision tree model. To explore the clinical significance of the established decision tree model in the early differential diagnosis of macrovascular complications of type 2 diabetes mellitus. Methods Fifty-five patients with macrovascular complications of type 2 diabetes mellitus and 84 patients with type 2 diabetes mellitus without macrovascular complications were enrolled in this study. Their protein profiles were obtained by WCX combined with MALDI-TOF-MS. Differences were analyzed and a decision tree model was established to expand the sample size, The reliability of the diagnostic model is verified by blind method. Results A diagnosis model of type 2 diabetes mellitus with large vascular complications consisting of 16 062.20, 31 883.60 and 7 986.76 differential protein peaks was established. The expanded sample size was 96.3% with a specificity of 100.0% . Conclusion The WCX magnetic beads combined with MALDI-TOF-MS technique can screen the differential protein peaks of patients with type 2 diabetes mellitus with macrovascular complications. The established decision tree model has certain clinical application prospects.