Objective:To explore the relationship between yin-deficiency constitution(YDC) and biochemical indexes by way of observing the endocrinal and immune functions in subjects with YDC.Methods:On the basis of epidemiological investigation,60 subjects with YDC and 50 with gentle constitution(GC) were selected according to the pertinent criteria.From each subject,8 mL of fasting venous blood was drawn at 8:00-9:00 in the morning,with the serum separated by centrifugation 3 000 r/min for 5 min and preserved at -70℃in a freezer.Serum levels of corticosterone,cortisol,adrenocorticotrophic hormone(ACTH),cyclic adenosine monophosphate(cAMP),cyclic guanosine monophosphate(cGMP),free triiodothyronine(FT3),free thyroxine (FT4),throtropic stimulation hormone,interleukin 1β(IL-1β) and interleukin 2(IL-2) were detected by doubleantibody sandwich ELISA;cAMP/cGMP ratio was calculated,and the difference between the two constitutions in terms of these indexes was analyzed.Results:Serum FT3 was 4.16±1.38 pmol/L in subjects with YDC,which was higher than that in subjects with GC(3.71±0.55 pmol/L),but levels of cortisol(124.58±45.36 ng/mL), ACTH(58.92±14.55 pg/mL),cGMP(66.00±18.02 pmol/mL) and FT4(12.33±3.12 pmol/L) in YDC were lower than those in GC(13.43±2.31 pmol/L),showing significant difference(P<0.05).Conclusion:YDC is related to some extent with the disturbances in the hypothalamus-pituitary-adrenal axis,hypothalamus-pituitary-thyroid axis,cyclic nucleoside system and immune function. Objective: To explore the relationship between yin-deficiency constitution (YDC) and biochemical indexes by way of observing the endocrine and immune functions in subjects with YDC. Methods: On the basis of epidemiological investigation, 60 subjects with YDC and 50 with gentle constitution ( GC) were selected according to the pertinent criteria. Fc each subject, 8 mL of fasting venous blood was drawn at 8: 00-9: 00 in the morning, with the serum separated by centrifugation 3000 r / min for 5 min and preserved at -70 ° C in a freezer. Levels of corticosterone, cortisol, adrenocorticotrophic hormone (ACTH), cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), free triiodothyronine (FT3), free thyroxine (FT4) , cAMP / cGMP ratio was was calculated, and the difference between the two constitutions in terms of these indexes was analyzed. Results: Serum FT3 was (interleukin 1β (IL-1β) and interleukin 2 4.16 ± 1.3 8 pmol / L in subjects with YDC, which was higher than that in subjects with GC (3.71 ± 0.55 pmol / L), but levels of cortisol (124.58 ± 45.36 ng / mL), ACTH (58.92 ± 14.55 pg / mL) cGMP (66.00 ± 18.02 pmol / mL) and FT4 (12.33 ± 3.12 pmol / L) in YDC were lower than those in GC (13.43 ± 2.31 pmol / L), showing significant difference to some extent with the disturbances in the hypothalamus-pituitary-adrenal axis, hypothalamus-pituitary-thyroid axis, cyclic nucleoside system and immune function.