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目的:分析测定葛根芩连汤中的14种药效组分,为建立其与临床疗效对应的质量标准提供技术和思路。方法:按照传统经方制备葛根芩连汤,采用高效液相色谱法(HPLC)对其药效组分进行测定。结果:葛根芩连汤中的14种药效组分,即葛根素、大豆苷、大豆苷元、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、盐酸小檗碱、盐酸巴马汀、盐酸药根碱、盐酸表小檗碱、盐酸黄连碱、甘草酸铵、甘草苷的含量分别为1.673 7%,0.153 3%,0.047 3%,0.448 0%,0.091 6%,0.012 3%,0.006 7%,0.095 5%,0.034 3%,0.043 8%,0.036 0%,0.048 3%,0.110 3%,0.158 0%。结论:所采用的HPLC法快速、简便,所测得的葛根芩连汤中的药效组分可作为其质量控制的科学指标。
OBJECTIVE: To analyze and determine 14 kinds of pharmacodynamic components in Gegen Qinlian Decoction, and to provide the technical and train of thought to establish the quality standard corresponding to clinical curative effect. Methods: Gegen Qinlian Decoction was prepared according to traditional prescription, and its pharmacodynamic components were determined by high performance liquid chromatography (HPLC). RESULTS: Fourteen pharmacodynamic components of Gegen Qinlian Decoction were puerarin, daidzin, daidzein, baicalin, baicalin, baicalein, wogonin, berberine hydrochloride, palmatine hydrochloride, The contents of jatrorrhizine hydrochloride, berberine hydrochloride, coptisine hydrochloride, ammonium glycyrrhizinate, and liquiritin were 1.673 7%, 0.153 3%, 0.047 3%, 0.448 0%, 0.091 6%, 0.012 3%, 0.006 7%, 0.095 5%, 0.034 3%, 0.043 8%, 0.036 0%, 0.048 3%, 0.110 3%, 0.158 0%. Conclusion: The HPLC method is rapid and simple. The measured pharmacodynamic components of Gegen Qinlian Decoction can be used as a scientific indicator of its quality control.