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目的利用流式细胞微球芯片捕获技术(CBA)检测慢加急性乙型肝炎肝衰竭(ACHBLF)患者辅助性T细胞1/2(Th1/Th2)分泌的细胞因子水平,探讨其在ACHBLF中变化的意义。方法收集33例ACHBLF、20例慢性乙型肝炎(CHB)患者血清及15名健康志愿者血清,CBA检测血清Th1/Th2细胞因子γ干扰素(IFN-γ)、白细胞介素2(IL-2)、白细胞介素4(IL-4)、白细胞介素6(IL-6)、白细胞介素10(IL-10)及肿瘤坏死因子α(TNF-α)水平,分析Th1/Th2细胞因子在ACHBLF患者血清中的变化。结果 ACHBLF患者外周血循环中TNF-α、IFN-γ、IL-4及IL-6的水平较CHB患者及健康对照者显著升高(P<0.05);ACHBLF患者血清中IL-2及IL-10与CHB组和健康对照组比较,差异无统计学意义(P>0.05)。ACHBLF患者血清中IL-4、IL-6、TNF-α及IFN-γ水平与总胆红素(TBil)、白蛋白(Alb)和凝血酶原活动度(PTA)间无明显相关性。结论 CBA可同时检测各种细胞因子,适合临床应用;ACHBLF患者TNF-α、IFN-γ、IL-4和IL-6的变化可能参与ACHBLF的发生和发展,未发现上述细胞因子与反映肝功能指标间的相关关系。
Objective To detect the levels of cytokines secreted by Th1 / Th2 cells in patients with chronic hepatitis B and liver failure (ACHBLF) by flow cytometry (CBA), and to investigate their changes in ACHBLF Meaning. Methods Serum levels of Th1 / Th2 cytokine IFN-γ, IL-2 and IL-2 were measured by CBA in 33 cases of ACHBLF, 20 cases of CHB and 15 healthy volunteers. , Interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 10 (IL-10) and tumor necrosis factor alpha (TNF- Serum changes in patients with ACHBLF. Results The levels of TNF-α, IFN-γ, IL-4 and IL-6 in patients with ACHBLF were significantly higher than those in CHB patients and healthy controls (P <0.05). The levels of IL-2 and IL- Compared with CHB group and healthy control group, the difference was not statistically significant (P> 0.05). The levels of IL-4, IL-6, TNF-α and IFN-γ in sera of patients with ACHBLF were not significantly correlated with the levels of total bilirubin (TBil), albumin (Alb) and prothrombin activity (PTA) Conclusion CBA can detect a variety of cytokines at the same time and is suitable for clinical application. The changes of TNF-α, IFN-γ, IL-4 and IL-6 in ACHBLF patients may be involved in the occurrence and development of ACHBLF. Correlation between indicators.