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目的:建立藏药桃儿七的HPLC指纹图谱,比较不同产地桃儿七成分含量差异,进行产地识别,综合评价桃儿七质量。方法:采用HPLC,Agilent Zorbax SB C18柱(4.6 mm×250 mm,5μm),乙腈-0.04%甲酸水为流动相梯度洗脱,流速1.0 mL·min-1,柱温30℃,对16批桃儿七样品进行指纹图谱测定,并使用主成分分析和聚类分析对指纹图谱进行产地识别和质量评价。结果:建立了桃儿七药材指纹图谱;对16批样品相似度比较,标定了29个共有峰;各产地样品成分组成基本相同,但是含量存在一定差异;对各样品进行化学模式识别,根据主成分得分,产自西藏的药材质量最好。结论:该方法能够用于桃儿七药材综合评价及质量控制。
OBJECTIVE: To establish the HPLC fingerprinting of Taoyuqi of Tibetan Medicine, and to compare the content differences of seven components of Taoyu from different areas to identify the origin and evaluate the quality of Taoyuan seven comprehensively. Methods: The mobile phase was eluted with a mobile phase of acetonitrile-0.04% formic acid on an Agilent Zorbax SB C18 column (4.6 mm × 250 mm, 5 μm) with a flow rate of 1.0 mL · min-1 at 30 ℃. Seven samples were measured fingerprinting, and the use of principal component analysis and cluster analysis of fingerprinting origin identification and quality evaluation. Results: The fingerprints of seven Chinese medicinal herbs were established. The similarity of 16 batches of samples was calibrated for 29 common peaks. The composition of the samples in each producing area was basically the same, but the contents were different. The chemical pattern of each sample was identified. Ingredients score, produced in Tibet, the best quality medicinal herbs. Conclusion: This method can be used for the comprehensive evaluation and quality control of seven Chinese medicinal herbs.