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目的:建立新疆伊贝母中西贝碱的含量测定方法,并对不同物候期伊贝母药材进行含量测定。方法:采用紫外分光光度法测定总生物碱类化合物的含量,以西贝碱为对照品,检测波长为411nm;结果:西贝碱检测浓度的线性范围为0.0178～0.0890mg.g-(1r=0.9985);平均加样回收率为100.3%,RSD=1.02%(n=9)。不同地区不同月份的伊贝母中总生物碱类化合物含量有一定差异。结论:本法专属性强,重现性好,结果准确可靠,可作为伊贝母的质量控制方法。4年生4月份采收的伊贝母中总生物碱类化合物含量比较高,本试验可为伊贝母的种植和采收的研究奠定基础。
Objective: To establish a method to determine the content of Elysem in Xinjiang, and to determine the content of Ebestein in different phenophases. Methods: The content of total alkaloids was determined by UV spectrophotometry. The detection wavelength was 411nm. The linear range of the concentration of cibasal was 0.0178 ~ 0.0890mg.g- (1r = 0.9985 ). The average recovery was 100.3% with RSD = 1.02% (n = 9). There were some differences in total alkaloid compounds in different regions in different months. Conclusion: This method is of high specificity, good reproducibility and accurate and reliable results, which can be used as a quality control method for 4-year-old in April collected the total alkaloids content in the Fritillaria, this experiment can lay the foundation for the study of planting and harvesting of Fritillaria.