The complex genetic architecture of quality traits has hindered efforts to modify seed nutrients in soybean.Genome-wide association studies were conducted for seed composition,including protein,oil,fatty acids,and amino acids,using 313 diverse soybean germplasm accessions genotyped with a high-density SNP array.A total of 87 chromosomal regions were identified to be associated with seed composition,explaining 8％-89％ of genetic variances.The candidate genes GmSAT1,AK-HSDH,SACPD-C,and FAD3A of known function,and putative MtN21 nodulin,FATB,and steroid-5-α-reductase involved in N2 fixation,amino acid biosynthesis,and fatty acid metabolism were found at the major-effect loci.Further analysis of additional germplasm accessions indicated that these major-effect loci had been subjected to domestication or mode breeding selection,and the allelic variants and distributions were relevant to geographic regions.We also revealed that amino acid concentrations related to seed weight and to total protein had a different genetic basis.This helps uncover the in-depth genetic mechanism of the intricate relationships among the seed compounds.Thus,our study not only provides valuable genes and markers for soybean nutrient improvement,both quantitatively and qualitatively,but also offers insights into the alteration of soybean quality during domestication and breeding.