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目的:了解骨保护素(osteoprotegerin,OPG)基因启动子区209G→A、245T→G基因多态性与绝经后妇女骨密度(bonemineraldensity,BMD)间的关系。方法:采用Pyrosequencing技术,检测上海地区201名绝经后妇女的OPG基因型。采用双能X线骨密度仪(DEXA)测量腰椎2-4(L2-4)、股骨颈、Ward三角和大转子的骨密度值(BMD)。结果:对OPG基因启动子区209和245位点进行检测共发现GG、GA和AA3种基因型,基因型频率分别为68.66%、29.85%和1.49%。GG型者的腰椎BMD明显高于GA和AA型者,且在校正年龄、体重指数(BMI)因素后,这种差异仍然存在(0.968±0.012比0.921±0.018,P<0.05)。结论:OPG基因是在中国绝经后妇女中进行骨量以及骨质疏松危险性研究的有用的遗传学标记。
OBJECTIVE: To investigate the relationship between 209G → A, 245T → G polymorphisms in promoter region of osteoprotegerin (OPG) gene and bone mineral density (BMD) in postmenopausal women. Methods: Pyrosequencing was used to detect OPG genotypes in 201 postmenopausal women in Shanghai. BMD of lumbar spine 2-4 (L2-4), femoral neck, Ward’s trochanter and greater trochanter were measured by dual energy X-ray absorptiometry (DEXA). Results: The genotypes of GG, GA and AA genotypes were detected in 209 and 245 loci of OPG gene. The genotype frequencies were 68.66%, 29.85% and 1.49%, respectively. The BMD of lumbar spine of GG type patients was significantly higher than those of GA and AA genotypes, and there was still a significant difference (0.968 ± 0.012 vs. 0.921 ± 0.018, P <0.05) after adjusting for age and body mass index (BMI). Conclusion: The OPG gene is a useful genetic marker for the study of bone mass and risk of osteoporosis in postmenopausal women in China.