对32个不同基因型大豆的幼胚进行不定胚诱导,30 d时统计分析初生胚诱导率、次生胚诱导率和诱导效率,经综合比较得出适合体细胞诱导不定胚的4种基因型为L155、垦丰16、绥农25和吉育91。然后以不定胚诱导率较高的L155为材料建立大豆悬浮培养不定胚繁殖体系,并用基因枪轰击的方法将GUS表达盒片段转入不定胚细胞团。X-gluc染色后显微观察到基因蓝色产物,说明GUS基因通过微弹轰击法导入大豆不定胚细胞并表达。 Adventitious embryos were induced in 32 immature embryos of different soybean genotypes, and primary embryo induction rate, secondary embryo induction rate and induction efficiency were statistically analyzed 30 days later. Four genotypes suitable for somatic cell induction of adventitious embryos For L155, Ken Feng 16, Sui Nong 25 and Ji Yu 91. Then, the adventitious embryos propagation system of soybean suspension culture was established by using L155 with higher adventitious embryo induction rate, and the GUS expression cassette fragment was transferred into adventitious embryonic cell mass by the bombardment method. The gene blue product was observed microscopically after X-gluc staining, indicating that the GUS gene was introduced into adventitious soybean embryo cells by microprojectile bombardment and expressed.