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对超高温(~80℃)自发热好氧堆肥工艺进行了研究,重点考察了堆肥过程中温度、含水率、氨氮浓度、氨气浓度、细菌丰度、污泥中可挥发性固体(VS)、种子发芽率(GI)的变化情况.并利用高通量测序考察了堆肥前后物料中微生物种群结构的变化情况.结果表明:堆肥过程中平均温度始终保持在70℃以上,峰值在82℃.含水率,VS随堆肥进行逐渐下降,最终含水率从44.17%降至31.94%,VS从45.08%降至40.25%.发酵最终减容40.95%,减量50.23%.细菌丰度在第一个周期中迅速减少,然后趋于稳定.NH4+-N在8.80~11.66mg/g干污泥内变化.浸提液稀释75倍时对种子发芽有明显促进作用.高通量测序分析显示:堆肥结束后,剩余污泥中的微生物93%以上裂解死亡.功能菌中主要为Firmicutes门中的Saccharomonospora属、Bacillus属、Geobacillus属、Oceanobacillus属,以及Actinobacteria门中的Actinomadura属、Thermobifida属,总量达83.09%.最终堆肥产品稳定无异味.“,”The dynamic law of temperature, moisture, ammonium ion, ammonia, bacteria abundance, volatile solid(VS) and seed germination index(GI) was studied during the ultra high temperature (~80℃) aerobic composting process. Meanwhile, the translocation of microbial community structure was revealed by high-throughput sequencing technology. The results showed that the average temperature in composting process remained above 70℃ and the maximum value was 82℃. Moisture and VS were decreasing from 44.17% to 31.94% and 45.08% to 40.25% during composting process, respectively. Noticeable volume (40.95%) and mass (50.23%) reduction was acquired. The abundance of total bacteria has declined dramatically in the first cycle and then maintained a stable level. The concentration of NH4+-N was varied between 8.80 and 11.66mg/gSS. The obvious facilitation of seed germination was verified at 75 times diluted liquid extract. High-throughput sequencing results showed that above 93% of bacterial cells were lysed after composting process. The functional bacteria was belonged to phyla of Firmicutes (genera ofSaccharomonospora,Bacillus,Geobacillus and Oceanobacillus) and Actinobacteria (genera ofActinomaduraandThermobifida), which account for 83.09% of the total bacteria abundance. Finally, the stable and odorlesscompost product was achieved.