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目的 探讨支气管哮喘患儿尿白三烯E4(LTE4)测定的临床意义。方法 采用竞争性酶联免疫吸附试验技术检测 2 8例哮喘患儿急性发作期和非急性发作期的尿LTE4水平 ,并与健康组儿童相比较 ;同时对哮喘患儿的尿LTE4与第 1秒用力呼气容积 (FEV1)及外周血嗜酸性粒细胞计数 (EC)进行相关性分析。结果 哮喘患儿非急性发作期尿LTE4比急性发作期明显下降 (P <0 0 1) ,但两期均明显高于健康组儿童 (均P <0 0 1)。急性发作期尿LTE4和FEV1呈负相关 (r =- 0 6 15 ,P <0 0 1) ,和EC无相关性 (r =0 16 3,P >0 0 5 )。结论 动态检测支气管哮喘患儿尿中LTE4水平 ,可能为将来的临床诊断和治疗提供有意义的参考指标。
Objective To investigate the clinical significance of urinary leukotriene E4 (LTE4) in children with bronchial asthma. Methods Urinary LTE4 levels in 28 asthmatic children during acute exacerbation and non-acute exacerbation were measured by competitive enzyme-linked immunosorbent assay (ELISA), and compared with healthy children. The forced expiratory volume (FEV1) and peripheral blood eosinophil count (EC) were analyzed. Results Compared with the acute exacerbation group, the urinary excretion of LTE4 in non-acute exacerbation of children with asthma was significantly decreased (P <0.01), but both of them were significantly higher than those of healthy children (all P <0.01). There was a negative correlation between LTE4 and FEV1 in acute exacerbation of urine (r = - 0 6 15, P 0 01) and no correlation with EC (r 0 163, P 0 05). Conclusion The dynamic detection of urinary LTE4 levels in children with bronchial asthma may provide meaningful reference for future clinical diagnosis and treatment.