以小麦抗逆相关蛋白TaMAPK2作为诱饵,利用酵母双杂交系统筛选小麦cDNA融合表达文库,获得候选蛋白TaE2。生物信息学分析表明,TaE2基因属于泛素结合酶UCE2基因家族成员。半定量RT-PCR结果表明TaE2基因在小麦根、茎、叶和种子等器官中均有表达,荧光定量PCR显示TaE2基因在干旱、高盐和ABA胁迫下均上调表达。利用原核表达获得GST-E2融合蛋白并使用蛋白标记亲和层析柱纯化。本文报道的小麦TaE2基因,为进一步分析泛素蛋白酶体途径在小麦逆境响应中的功能和机制打下基础。 Using wheat TaqMAPK2 as a bait, yeast two-hybrid system was used to screen the cDNA fusion expression library of wheat to obtain the candidate protein TaE2. Bioinformatics analysis showed that the TaE2 gene is a member of the UCE2 gene family of ubiquitin-binding enzymes. Semi-quantitative RT-PCR results showed that TaE2 gene was expressed in wheat roots, stems, leaves and seeds and other organs. Fluorescence quantitative PCR showed that TaE2 gene was up-regulated under drought, high salt and ABA stress. GST-E2 fusion protein was obtained using prokaryotic expression and purified using protein-labeled affinity chromatography. The TaE2 gene of wheat reported in this paper laid the foundation for further analysis of the function and mechanism of ubiquitin proteasome pathway in wheat response to stress.